ABSTRACT
<p><b>OBJECTIVE</b>To identify the anti-inflammatory effects of artificial musk aqueous extract(AME)on lipopolysaccharide-stimulated cytokines secreted or released by RAW264.7 cells.</p><p><b>METHODS</b>Cytokines including interleukin(IL)-6,IL-10,and tumor necrosis factor Α were determined using cytokine enzyme-linked immunosorbent assay kits.</p><p><b>RESULT</b>Compared with model group,the levels of major cytokines such as tumor necrosis factor Α,IL-6,and IL-10 significantly decreased in different AME groups in a dose-dependent manner.</p><p><b>CONCLUSION</b>In lipopolysaccharide-stimulated RAW264.7 macrophages,AME can remarkably inhibit the release of inflammatory cytokines and thus exerts its anti-inflammatory effects.</p>
Subject(s)
Animals , Anti-Inflammatory Agents , Pharmacology , Cell Line, Tumor , Cytokines , Metabolism , Enzyme-Linked Immunosorbent Assay , Fatty Acids, Monounsaturated , Pharmacology , Inflammation Mediators , Metabolism , Interleukin-6 , Metabolism , Lipopolysaccharides , Macrophages , Tumor Necrosis Factor-alpha , MetabolismABSTRACT
<p><b>OBJECTIVE</b>To control the quality of Xihuangwan by improving the qualitative detection of artificial musk inside this pill.</p><p><b>METHOD</b>The qualitative detection of artificial musk was carried out by gas chromatography-mass spectrometery(GC-MS).</p><p><b>RESULT</b>The established GC-MS successfully detected artificial musk in Xihuangwan.</p><p><b>CONCLUSION</b>GC-MS is reliable,accurate,and practical in the identification of artificial must inside Xihuangwan.</p>
Subject(s)
Drugs, Chinese Herbal , Chemistry , Fatty Acids, Monounsaturated , Chemistry , Gas Chromatography-Mass SpectrometryABSTRACT
<p><b>OBJECTIVE</b>To evaluate the safety of artificial musk by determining its content of five hazard heavy metals.</p><p><b>METHOD</b>The contents of Pb, Cd,As,Hg,and Cu in artificial musk were detected using inductively coupled plasma-mass spectrometry.</p><p><b>RESULT</b>The contents of Pb,Cd,As,Hg,and Cu was less than 0.35,0.05,0.30,0.20,and 4.50 mg/kg in artificial musk.</p><p><b>CONCLUSION</b>Artificial musk has low contents of heavy metals and therefore meets the safety and quality requirments.</p>